DEPARTMENT INSTRUMENTATION

The UST Chemistry Department has excellent instrumentation facilities for analysis of most kinds of samples.  In addition to common chromatographic and spectroscopic instruments, we have many instruments normally found only in specialized labs.  In addition to what is included here, individual research groups have specialized instrumentation such as lasers, lock-in amplifiers, current amplifiers, additional chromatographic equipment and thermal cyclers.  To see pictures of the actual instruments click on the instruments name.

 

  1. NMR. Our NMR is a JEOL ECS 400 MHz NMR Spectrometer.  It has a multinuclear probe with automatic tuning.  It is also equipped with an automatic 24 position sample changer.  It has variable temperature capabilities for dynamic NMR experiments.

    The magnet has gradient shimming that gives extraordinary line shapes on every spectrum.  All 1-D and 2-D experiments can be fully automated.  The instrument is controlled by Delta software that runs on a 27 inch iMac.  The instrument can also be controlled from any office computer.
  2. PCR/DNA Sequencer.  We purchased a BioRad iQ5 in 2008.  This powerful instrument has enhanced our biochemistry research capabilities.  
  3. LC-MS/MS.  UST Chemisty has a Varian 212 LC with autosampler interfaced with a Varian 320 triple quadrupole mass spectrometer.  The instrument has both electrospray (ESI) and atmospheric pressure chemical ionization (APCI) sources.  This instrument was purchased with funds received from and NSF MRI grant. 
  4. High Resolution Mass Spectrometry.  Through a generous donation from 3M, we have a Micromass QTof2 with an electrospray ion source.
  5. Gas Chromatographic Mass Spectrometry. The department has a Hewlett-Packard 5972 mass spectrometer interfaced to a 5890 GC.  The detector is capable of electron impact and chemical ionization. 
  6. Other Gas Chromatography.  We have two other gas chromatographic systems:    i) A Shimadzu model GC14-A with a flame ionization detector ii) A Hewlett Packard 5890 with a plasma discharge helium ionization detector (PDHID) 
  7. High Performance Liquid Chromatography. We have 4 HPLC systems for general use: i) AShimadzu model LC-10AT with a model SPD-10A multiwavelength UV detector  and  a model ELSD-LT evaporative light scattering detector. ii) A Waters model 660E HPLC ternary gradient system with a model 990 PDA  diode array UV/visible detector. iii) A Viscotek GPC max gel permeation chromatograph with a model TDA 302 Triple Array Detector iv) A fourth HPLC instrument with a fraction collector resides in the biochemistry lab. It has been used primarily for protein separations and preparative work. 
  8. Capillary Electrophoresis. This instrument is a Beckman Pace 5010 model with both UV absorption and laser induced fluorescence detectors. It has an auto-sample tray. (We also have several different types of standard slab gel electrophoresis instruments.) 
  9. Fourier Transform Infrared Spectrometer. We purchased a new Perkin Elmer Spectrum RXI FTIR instrument in 2005. 
  10. Probe IR.  Through a generous donation from Imation, the Department has a ReactIR1000 from Applied Systems.  This system contains a mobile probe that can be placed inside reaction vessels to follow the progress of a reaction by IR. 
  11. Raman Spectrometry.  The department acquired a DaltaNu Advantage 200A Raman Spectrometer in 2005. 
  12. Electroanalysis. The Department has a Bioanalytical Systems Electrochemistry station for doing all sorts of voltammetry and coulometry. We have ultra-micro electrodes and the low current module and a high current module for doing bulk electrolysis and a homemade rotated disk system as well. 
  13. Fluorescence Spectrometry.  Our spectrofluorometer is a Fluoromax from Spex. It is capable of recording excitation, emission or synchronous fluorescence spectra. We also have a flow cell for this instrument. 
  14. Atomic Absorption Spectroscopy.  In 2007, we purchased a new Varian model 55B atomic absorption spectrophotometer with multielement lamps allowing analysis of Ca, Mg, Fe, Zn, Pb, Co, and Cu. 
  15. UV/Visible absorption spectrometry. We have a diode array instrument (HP model 8452) and two rapid scanning Shimadzu dual beam instruments. There are also many desk-top simple photometers in various labs as well. 
  16. Thermal Analysis:  We recently purchased two instruments.  A TA model DSC Q100 differential scanning calorimeter and a TA model TGA Q500 thermal gravimetric analyzer. 
  17. Centrifuges. We have a Sorvall RC-5B+ refrigerated centrifuge with several different sizes of rotors. We also have three Sorvall Microcentrifuges. 
  18. Glovebox: For working with air- or moisture-sensitive compounds, we have a stainless steel glovebox. 
  19. Microwave Reactor: The department has a CEM Discover microwave reactor with autosampler.
  20. Handheld Fluorescence Spectrometer:
  21. Other Synthetic Tools:  Our synthetic research lab contains a Biotage Initiator Microwave Reactor, as well as two Biotage Isolera One automated fraction collector/purification systems. 
  22. Crystallographic facilities. The Department, through collaboration with the X-ray Crystallographic Laboratory of the University of Minnesota, obtains low-temperature X-ray diffraction data sets through the use of the Bruker-AXS CCD diffractometers of the XCL. These data sets are then transferred to a UST Chemistry Department laboratory for structure solution and refinement. For this purpose, the Department has acquired its own copy of the SHELXTL crystallographic software package. The Department also maintains up-to-date releases of the Cambridge Structural Database, useful for structure searches and molecular geometry analyses.