Microbiology

June 22, 2012 / By: Jayna L. Ditty PD.D
Researcher: Jayna Ditty, Ph.D.

Professional Interests

  • Molecular mechanism of the Synechococcus elongatus prokaryotic circadian clock

  • Photic entrainment of the Synechococcus elongatus circadian clock

  • Efficacy of novel antimicrobial compounds

  • Chemotaxis toward aromatic hydrocarbons in Pseudomonads

Publications & Presentations

Names in bold  or * denote student research collaborators.

  • Ditty J.L., Williams K.M.*, Keller M.M.*, Chen G.Y., Liu X., Parales R.E. (in press) Integrating Grant-funded Research into the Undergraduate Biology Curriculum Using IMG-ACT. Biochemical and Molecular Biology Education.Nichols N.N, Lunde T.A.*, Graden K.C.*, Hallock K.A.*, Kowalchyk C.K.*, Southern R.M.*, Soskin E.J.*, Ditty J.L. (2012) Chemotaxis to furan compounds by furan-degrading Pseudomonas strains. Applied and Environmental Microbiology 78:6365-6368.
  • Wammer, K.M., Slattery, M.T., Stemig, A.M., and Ditty, J.L. (2011). Analysis of the antibacterial activity of tetracycline’s photoproducts. In review: Chemosphere.
  • Mackey, S.R., Golden, S.S., and Ditty, J.L. (2011). The itty-bitty time machine: Genetics of the cyanobacterial circadian clock. In S. Brody (Ed.) Advances in Genetics, vol. 74. Elsevier
  • Ditty, J.L. et al. (2010) Faculty Incorporating Genomics and Bioinformatics Across the Life Sciences Curriculum: Development and Implementation of the Integrated Microbial Genomes Annotation Collaboration Toolkit. PLoS Biol 8(8): e1000448. doi:10.1371/journal.pbio.1000448
  • R.E. Parales and Ditty, J.L. (2010) Chemotaxis. In K.N. Timmis (Ed.), Handbook of hydrocarbon and lipid microbiology. Berlin: Springer-Verlag.
  • R.E. Parales and Ditty, J.L. (2010) Substrate transport. In K.N. Timmis (Ed.), Handbook of hydrocarbon and lipid microbiology. Berlin: Springer-Verlag.
  • Ditty, J.L., Nichols, N.N., and Parales, R.E. (2010) Measurement of hydrocarbon transport in bacteria. In K.N. Timmis (Ed.), Handbook of hydrocarbon and lipid microbiology. Berlin: Springer-Verlag.
  • Ditty, J.L., and Rolnick, P.A. (2010) Keeping faith: evolution and theology. Logos: A Journal of Catholic Thought and Culture. St. Paul: University of St. Thomas
  • Ditty, J.L., Mackey, S.R., and Johnson, C.H. (Eds.) (2009). Bacterial Circadian Programs. Berlin: Springer-Verlag.
  • Ditty, J.L. and Mackey, S.R. (2009). Classic circadian characteristics: historical perspective and properties relative to the Synechococcus elongatus PCC 7942 model. In J.L. Ditty, S.R. Mackey, & C.H. Johnson (Eds.) Bacterial Circadian Programs. Berlin: Springer-Verlag.
  • Mackey, S.R., Ditty, J.L., Zeidner, G., Chen, Y., and Golden, S.S. (2009). Mechanisms for entraining the cyanobacterial circadian clock system with the environment. In J.L. Ditty, S.R. Mackey, & C.H. Johnson (Eds.) Bacterial Circadian Programs. Berlin: Springer-Verlag.
  • Ebner, D., Culhane, J., Winkelman, T., Haustein, M.D., Ditty, J.L., and Ippoliti, J.T. (2008). Synthesis of a novel oxazolidinone antimicrobial agent. Bioorg. Med. Chem. 16:2651-2656.
  • Parales R.E., Parales J.V., Pelletier D.A., and Ditty J.L. (2008). Diversity of microbial toluene degradation pathways. Adv. Appl. Microbiol. 64:1-73.
  • Parales, R.E., Ju, K.-S., Rollefson, J.B., and Ditty, J.L. (2008). Bioavailability, transport, and chemotaxis towards organic pollutants. In E. Diaz (Ed.), Microbial Biodegradation: Genomics and Molecular Biology. Norfolk, UK: Caister Academic Press.
  • Mackey, S.R., Ditty, J.L., Clerico, E.M., and Golden, S.S. (2007). Detection of rhythmic bioluminescence from luciferase reporters in cyanobacteria. In E. Rosato (Ed.), Circadian Rhythms: Methods and Protocols. In J.M. Walker (Series Ed.) Methods in Molecular Biology. Totowa, New Jersey: Humana Press.
  • Clerico, E.M., Ditty, J.L., and Golden, S.S. (2007). Specialized techniques for site-directed mutagenesis in cyanobacteria. In E. Rosato (Ed.), Circadian Rhythms: Methods and Protocols. In J.M. Walker (Series Ed.) Methods in Molecular Biology. Totowa, New Jersey: Humana Press.
  • Ditty, J.L., Kelley, D., Lorah, P., Steyermark, A., Werner, R., and Zimmer, K. (2007). Cross-departmental implementation of GIS at a mid-sized undergraduate University. CUR Quarterly 27:120-123.
  • Parales, R.E., and Ditty, J.L. (2005). Laboratory evolution of catabolic enzymes and pathways. Current Opinion in Biotechnology 16:315-325.
  • Ditty, J.L., Canales, S.R., Anderson, B.E., Williams, S.B., and Golden, S.S. (2005). Stability of the Synechococcus elongatus PCC 7942 circadian clock under directed anti-phase expression of the kai genes. Microbiology 151:2605-2613.
  • Canales, S.R., J.L. Ditty, E.M. Clerico, and S.S. Golden. (2005) Detection of rhythmic bioluminescence from luciferase reporters in cyanobacteria. Methods in Molecular Biology(in press)
  • Ditty, J.L., S.R. Canales, B.E. Anderson, S.B. Williams, and S.S. Golden. (2005) Stability of the Synechococcus elongatus PCC 7942 circadian clock under directed anti-phase expression of the kai genes. Microbiology 151: 2605-2613.
  • Ditty, J.L., S.B. Williams, and S.S. Golden. (2003) A cyanobacterial circadian timing mechanism. Annual Review Genetics 37: 513-43.
  • Nair, U., J.L. Ditty, Y. Xu, H. Min, and S.S. Golden. (2002) Roles for sigma factors in global circadian regulation of the cyanobacterial genome. Journal of Bacteriology184:3530-3538.
  • Ditty, J.L., and C.S. Harwoood. (2002) Charged amino acids conserved in the aromatic acid/H(+) symporter family of permeases are required for 4-hydroxybenzoate transport by PcaK from Pseudomonas putidaJournal of Bacteriology 184:1444-1448.
  • Parales, R.E., J.L. Ditty, and C.S. Harwood. (2000) Toluene-degrading bacteria are chemotactic towards the environmental pollutants benzene, toluene, and trichloroethylene.  Applied and Environmental Microbiology  66:4098-4104.
  • Ditty, J.L., and C.S. Harwood. (1999) Conserved cytoplasmic loops are important for both the transport and chemotaxis functions of PcaK, a 12 membrane-spanning protein fromPseudomonas putidaJournal of Bacteriology 181: 5068-5074.
  • Ditty, J.L., A.C. Grimm, and C.S. Harwood. (1988) Identification of a chemotaxis gene region from Pseudomonas putidaFEMS Microbiology Letters 159: 267-273.
  • Harwood, C.S., N.N. Nichols, M.-K. Kim, J.L. Ditty, and R.E. Parales. (1994) Identification of the pcaRKF gene cluster from Pseudomonas putida: Involvement  in chemotaxis, biodegradation, and transport of 4-hydroxybenzoate. Journal of Bacteriology176: 6470-6488.

Grant Awards

  • LI-COR Genomics Education Matching Fund Instrumentation Grant: LI-COR Model 4300 Analysis System (2010)
  • DOE JGI Undergraduate Research Program in Microbial Genome Annotation. Pilot faculty collaborator (2008)
  • Merck/AAAS Undergraduate Science Research Program.  Project 3: Synthesis and testing of novel antimicrobial compounds (2007-2009)
  • National Science Foundation. MCB-0919930. Bacterial chemotaxis to Aromatic Hydrocarbons and Related Pollutants. (2009)
  • Starter Grant, National Science Foundation MCB-0329366.  Project title: Circadian Rhythms in Cyanobacteria.Research Assistance Grant, University of St. Thomas Faculty Development program.  Project title: How do bacteria tell time? Characterizing Regulatory Elements in Circadian Clock Genes in Cyanobacteria.

Student Researchers

Current

Effect of photoperiod on circadian rhythms in Synechococcus elongatus PCC 7942

  • Michael Grahl
  • Beth Cink

Chemotaxis of Psuedomonas putida F1 to amino acids

  • Megan Keller
  • Kayla Williams

Chemotaxis of Psuedomonas putida F1 to aromatic acids

  • Ashley Hagerty

Chemotaxis of Pseudomonads to furan compounds

  • Ellie Soskin (Breck High school)

 

Chemotaxis of Psuedomonas putida F1 to phenylacetate

  • Ben Schneider
  • Stacy Ngwesse

Past

Circadian Rhythm Projects

  • Breanne Anderson
  • Christina Bye
  • Ann Defnet
  • Kelly Delaney
  • Joe Dubis
  • Chelsea Grolla
  • Christine L’Abbe
  • Tamara Johnson
  • Tucker Johnson
  • Bethany Rhein
  • Amy Sobolewski

Chemotaxis Projects

  • Solomon Anderson
  • Maria Bye
  • Kevin Graden
  • Kate Hallock
  • Allison Korman
  • Cara Kowalchyk (Breck High School)
  • Katie Kremer
  • Brent Larson
  • Tristan Lunde
  • Janet Rollefson
  • Rebecca Southern (Breck High School)
  • Christopher Stefonowicz

Synthesis and activity of new antimicrobials

  • Lucy Gansebom
  • Mitchell Haustein
  • Tyler Winkelman