Microbiology

June 22, 2012 / By: Jayna L. Ditty PD.D
Researcher: Jayna Ditty, Ph.D.

Professional Interests

◦ Chemotaxis toward aromatic hydrocarbons in Pseudomonas species

◦ Use of Bioinformatic tools in the undergraduate curriculum

◦ Photic entrainment of the Synechococcus elongates circadian clock

◦ Efficacy of novel antimicrobial compounds

 

Ditty lab summer 2014

Research students, Summer 2014

 Back row: Samuel Madden, Siddarth Eswarachari (Breck High school) Taylor Severson, Cory Jensen

Front row: Moira Southern (Breck High school) Katie Elert, Victoria Maksimova

 

Publications (since 2003)

 

Primary Articles  (Names in bold indicate student collaborators.) 

Parales, R.E, Nesteryuk, V., and Ditty, J.L. (2014). Chemotaxis to nicotinic acid and identification of the methyl-accepting chemotaxis protein in Pseudomonas putida F1. Under review at Microbiology

 

Parales, R.E, Luu, R.A., Chen, G.Y., Liu, X., Wu, V., Lin. P., Hughes, J.G., Nesteryuk, V., Parales, J.V., and Ditty, J.L. (2013). Pseudomonas putida F1 has multiple chemoreceptors with overlapping specificity for organic acids. Microbiology 159:1086-1096.

 P putida ditty research

 

Luu, R.A., Schneider, B.J., Ho, C.C., Nesteryuk, V., Ngwesse, S.E., Liu, X., Parales, J.V., Ditty, J.L., and Parales, R.E. (2013). Taxis to phenylacetic acid by Pseudomonas putida F1 is mediated by the energy taxis receptor Aer2. Applied and Environmental Microbiology 79:2416-2423.

 

Nichols, N.N, Lunde, T.A., Graden, K.C., Hallock, K.A., Kowalchyk, C.K., Southern, R.M., Soskin, E.J., and Ditty, J.L. (2012). Chemotaxis to furan compounds by furan-degrading Pseudomonas strains. Applied and Environmental Microbiology 78:6365-6368.

 

Wammer, K.M., Slattery, M.T., Stemig, A.M., and Ditty, J.L. (2011). Analysis of the antibacterial activity of tetracycline’s photoproducts. Chemosphere 85:1505-1510.

 

Ebner, D., Culhane, J., Winkelman, T., Haustein, M.D., Ditty, J.L., and Ippoliti, J.T. (2008). Synthesis of a novel oxazolidinone antimicrobial agent. Bioorganic and Medicinal Chemistry 16:2651-2656.

 

Ditty, J.L., Canales, S.R., Anderson, B.E.,Williams, S.B., and Golden, S.S. (2005). Stability of the Synechococcus elongatus PCC 7942 circadian clock under directed anti-phase expression of the kai genes. Microbiology 151:2605-2613.

 

Nair, U., Ditty, J.L., Xu, Y., Min, H., and Golden, S.S. (2002). Roles for sigma factors in global circadian regulation of the cyanobacterial genome. Journal of Bacteriology 184:3530-3538.

 

Ditty, J.L., and Harwood, C.S. (2002). Charged amino acids conserved in the aromatic acid/H(+) symporter family of permeases are required for 4-hydroxybenzoate transport by PcaK from Pseudomonas putida. Journal of Bacteriology 184:1444-1448.

 

Parales, R.E., Ditty, J.L., and Harwood, C.S. (2000). Toluene-degrading bacteria are chemotactic towards the environmental pollutants benzene, toluene, and trichloroethylene.  Applied and Environmental Microbiology 66:4098-4104.

 

Ditty, J.L., and Harwood, C.S. (1999). Conserved cytoplasmic loops are important for both the transport and chemotaxis functions of PcaK, a 12 membrane-spanning protein from Pseudomonas putida. Journal of Bacteriology 181:5068-5074.

 

Ditty, J.L., Grimm, A.C., and Harwood, C.S. (1998). Identification of a chemotaxis gene region from Pseudomonas putida. FEMS Microbiology Letters 159:267-273.

 

Harwood, C.S., Nichols, N.N., Kim, M.-K., Ditty, J.L., and Parales, R.E. (1994). Identification of the pcaRKF gene cluster from Pseudomonas putida: Involvement in chemotaxis, biodegradation, and transport of 4-hydroxybenzoate. Journal of Bacteriology 176:6470-6488.

 

    Edited Book

Ditty, J.L., Mackey, S.R., and Johnson, C.H. (Eds.) (2009). Bacterial Circadian Programs. Berlin: Springer-Verlag.

 

    Book Chapters

Mackey, S.R., Golden, S.S., and Ditty, J.L. (2011). The itty-bitty time machine: Genetics of the cyanobacterial circadian clock. In S. Brody (Ed.) Advances in Genetics 74:13-53.

 

Parales, R.E. and Ditty, J.L. (2010). Chemotaxis. In K.N. Timmis (Ed.), Handbook of Hydrocarbon and Lipid Microbiology. Berlin: Springer-Verlag.

 

Parales, R.E. and Ditty, J.L. (2010). Substrate transport. In K.N. Timmis (Ed.), Handbook of Hydrocarbon and Lipid Microbiology. Berlin: Springer-Verlag.

 

Ditty, J.L. and Mackey, S.R. (2009). Classic circadian characteristics: historical perspective and properties relative to the Synechococcus elongatus PCC 7942 model. In J.L. Ditty, S.R. Mackey, & C.H. Johnson (Eds.) Bacterial Circadian Programs. Berlin: Springer-Verlag.

 

Mackey, S.R., Ditty, J.L., Zeidner, G., Chen, Y., and Golden, S.S. (2009). Mechanisms for entraining the cyanobacterial circadian clock system with the environment. In J.L. Ditty, S.R. Mackey, & C.H. Johnson (Eds.) Bacterial Circadian Programs. Berlin: Springer-Verlag.

 

Parales R.E., Parales J.V., Pelletier D.A., and Ditty J.L. (2008). Diversity of microbial toluene degradation pathways. Advanced and Applied Microbiology. 64:1-73.

 

Parales, R.E., Ju, K.-S., Rollefson, J.B., and Ditty, J.L. (2008). Bioavailability, transport, and chemotaxis towards organic pollutants. In E. Diaz (Ed.), Microbial Biodegradation: Genomics and Molecular Biology. Norfolk, UK: Caister Academic Press.

 

Parales, R.E., and Ditty, J.L. (2005). Laboratory evolution of catabolic enzymes and pathways. Current Opinion in Biotechnology 16:315-325.

 

Ditty, J.L., Williams, S.B., and Golden, S.S. (2003). A cyanobacterial circadian timing mechanism. Annual Review Genetics 37:513-43.

 

    Methods Book Chapters

Ditty, J.L., Nichols, N.N., and Parales, R.E. (2010). Measurement of hydrocarbon transport in bacteria. In K.N. Timmis (Ed.), Handbook of Hydrocarbon and Lipid Microbiology. Berlin: Springer-Verlag.

 

Mackey, S.R., Ditty, J.L., Clerico, E.M., and Golden, S.S. (2007). Detection of rhythmic bioluminescence from luciferase reporters in cyanobacteria. In E. Rosato (Ed.), Circadian Rhythms: Methods and Protocols. In J.M. Walker (Series Ed.) Methods in Molecular Biology. Totowa, New Jersey: Humana Press.

 

Clerico, E.M., Ditty, J.L., and Golden, S.S. (2007). Specialized techniques for site-directed mutagenesis in cyanobacteria. In E. Rosato (Ed.), Circadian Rhythms: Methods and Protocols. In J.M. Walker (Series Ed.) Methods in Molecular Biology. Totowa, New Jersey: Humana Press.

 

    Pedagogical Publications

Ditty, J.L., Williams, K.M., Keller, M.M., Chen, G.Y., Liu, X., and Parales, R.E. (2013). Integrating Grant-funded Research into the Undergraduate Biology Curriculum Using IMG-ACT. Biochemical and Molecular Biology Education 41:16-23.

 

Ditty, J.L. et al. (2010). Faculty Incorporating Genomics and Bioinformatics Across the Life Sciences Curriculum: Development and Implementation of the Integrated Microbial Genomes Annotation Collaboration Toolkit. PLoS Biology 8(8): e1000448. doi:10.1371/journal.pbio.1000448

 

Ditty, J.L., Kelley, D., Lorah, P., Steyermark, A., Werner, R., and Zimmer, K. (2007). Cross-departmental implementation of GIS at a mid-sized undergraduate University. CUR Quarterly 27:120-123.

 

    Other Publications

Ditty, J.L., and Rolnick, P.A. (2010). Keeping faith: Evolution and Theology. Logos: A Journal of Catholic Thought and Culture. St. Paul: University of St. Thomas

 

ditty research

 

Grants and Awards

Applied and Environmental Microbiology:

   Editorial board member (2014-2016)

Microbial Genome Annotation Network (MGAN):

   Governing board member (2012 to present)

LI-COR Genomics Education Matching Instrumentation Grant:

   LI-COR Model 4300 Analysis System (2010)

DOE-JGI Undergraduate Research Program in Microbial Genome Annotation:

   Pilot faculty collaborator (2008)

Merck/AAAS Undergraduate Science Research Program:

Project 3: Synthesis and testing of novel antimicrobial compounds (2007-2009)

National Science Foundation MCB-0919930:

Bacterial chemotaxis to Aromatic Hydrocarbons and Related Pollutants. (2009-2012)

National Science Foundation Starter Grant MCB-0329366:

   Circadian rhythms in cyanobacteria. (2003)

National Science Foundation Post-Doctoral Fellowship in Microbial Biology DBI-0074386:

Characterization of a second circadian oscillator in Synechococcus sp. PCC 7942. (2000-2002)