Cell Biology

November 25, 2013 / By: Jennifer Cruise, PH.D.

Professional Interests

  • The role of monomeric GTPases in the regulation of cell attachment, migration, and proliferation.
  • Genomic structure of mammalian gene promoters.
  • Applying the tools of cell biology to questions in population biology.

 

Current Projects

  • Effect of Rap1 proteins on cell proliferation and programmed cell death.
  • Identification of promoter control regions of the human rap1A and rap1B genes.
  • Using mitochondrial function to test fitness predictions of mating system alternatives in an invasive freshwater snail.

Publications & Presentations (Selected)

Student names are in bold.

  • A. De, S.L. Donahue, A. Tabah, N.E. Castro, N. Mraz, J.L. Cruise, and C.Campbell (2006) A novel interaction of nucleolin with Rad51. BIOCHEM. BIOPHYS. RES. COMMUN. 344:206-214.
  • J.L. Cruise (2001)- Minnesota Academy of Science Annual Meeting      St. Paul, MN. "Cell-cycle related expression of Rap1, a ras family member and potential inhibitor of proliferation."  related expression of Rap1, a ras family member and potential inhibitor of proliferation."
  • J. Broten, G. Michalopoulos, B. Petersen, and J.L. Cruise (1999) Adrenergic stimulation of hepatocyte growth factor expression.  BIOCHEM. BIOPHYS. RES. COMMUN. 262(1):76-79.
  • J.L. Cruise,  M.P. Rafferty, and M.M. Riehle  (1997) Cell-cycle-regulated expression of Rap1 in regenerating liver.  BIOCHEM. BIOPHYS. RES. COMMUN. 230: 578-581.
  • B. Thyagarajan,  J.L. Cruise, and C. Campbell (1996) Elevated levels of homologous DNA recombination activity in the regenerating rat liver.  SOMATIC CELL AND MOLECULAR GENETICS. 22(1): 31-39.


Abstracts and Student Presentations 

  • Jessica Broten, 1999. "Alpha adrenergic regulation of HGF expression in MRC-5 fibroblasts." National Conference on Undergraduate Research, Rochester, NY. Also presented at  Minnesota Academy of Science, St. Paul, MN. Mayo Award Winner.
  • William Cross, 2000. "Rap1A basal level importance and regulation in multiple cell types," Minnesota Academy of Science, Moorhead, MN, April 2000.
  • Amy Gaviglio, 2005. “The Effect of Rap1 on the small G-proteins Rac and cdc42, and on cell cycle control.” Iowa Academy of Science, Cornell College, Mount Vernon, IA. Johnson Award Winner.
  • Michelle Gorman, 2000. "IgY antibody against mammalian nucleolin, a protein implicated in DNA recombination." Tri-Beta Regional Meeting, Bethel College, Arden Hills, MN; Second Place Poster Award Winner, April 2000.
  • Matt Hallman and Laura Smid, 1998. "BMK1 activation as a result of shear stress," Minnesota Academy of Science, Winona, MN.
  • Steve Harrold, 1998. “Norepinephrine causes an increase in HGF concentration in MRC-5 media,”  Minnesota Academy of Science, Winona, MN.
  • Maria Hindt, 2009. " Promoter Characterization of rap1, a possible tumor supressor gene," Minnesota Academy of Science, St. Paul, MN. Winchell Award Winner.
  • Sarah Hoper, 1998. “The role of phophoinositide 3-Kinase in hepatocyte DNA replication  stimulated by norepinephrine,” Minnesota Academy of Science, Winona, MN. Winchell Award Winner.
  • Julie Horbul, 2002. "Exploring the relationship between Rap1 and Rho, small G-Proteins implicated in cell cycle control," Tri-Beta Regional Meeting, Bethel College,  College, Arden Hills, MN. 
  • Sarah Hyatt , 2007. "The effect of Rap1 on programmed cell death." Minnesota Academy of Science Annual Meeting, St. Paul, MN.
  • Keith Hultman, 2001. "Development of microsatellite DNA markers for Aquilegia canadensis," Tri-Beta Regional Meeting, St. Mary's University, Winona, MN.
  • Jessica Janisch, 2001." Circadian rhythm studies: Genotyping of mPer2 knockout mice and in situ hybridization for determination of per gene expression," Minnesota Academy of Science Annual Meeting, St. Paul, MN.
  • Andrea Johnson, 2006. " Is there a role for Rap1 in the mammalian cell cycle?" Minnesota Academy of Science Annual Meeting, St. Paul, MN. Winchell Award Winner
  • Nikos Katopodis.1990. “Levels of  rap1a mRNA in liver tissue during the process of liver regeneration.” Minnesota Academy of Science Annual Meeting, St. Paul, MN.
  • Daniel Kolar, 2006. "Effects of Rap1 on cellular migration and adhesion." Minnesota Academy of Science  Annual Meeting, St. Paul, MN.
  • Jennifer Kuzma. 1990. " Virus-mediated cell fusion for delivery of monoclonal antibodies to intracellular targets." ACTC Annual Biology Undergraduate Research Symposium, St. Paul, MN
  • Tinna Laughlin. 1991.  “Uncoupling of the alpha1adrenergic receptor and DNA synthesis.”  Minnesota Academy of Science Annual Meeting, St. Paul, MN; National Conference on  Undergraduate Research, Minneapolis, MN.
  • Karen Lommel, 1994."The role of cyclic AMP in insulin-stimulated DNA synthesis." 8th National Conference on Undergraduate Research, Kalamazoo, MI.
  • Ryan McAdams, 1994." Indomethacin does not inhibit the stimulatory effect of norepinephrine on DNA synthesis in primary cultures of rat hepatocytes." North Central Region District 3 Tri Beta Convention, St. Paul, MN
  • Maureen McDonnell, 1994. "Norepinephrine-induced down regulation of the epidermal growth factor receptor: GTP-dependent and independent effects." Argonne National Laboratory Undergraduate Research Symposium, Argonne, IL. (Also 1st place at 1994 North Central Region District 3 Tri Beta Convention, St. Paul, MN.)
  • Rebecca Mooney, 2001. "Development of microsatellite DNA markers for Aquilegia canadensis," Minnesota Academy of Science Annual Meeting, St. Paul, MN.
  • Naomi, Mraz, 1999. "The role of nucleolin in homologous DNA recombination," Minnesota Academy of Science, St. Paul, MN. Winchell Award Winner.
  • Yvonne  Mudek, 1990. "Cyclooxygenase products as potential mediators of enhanced DNA synthesis in cultured rat hepatocytes." 7th Annual Associated Colleges of the Twin Cities Biology Undergraduate Research Symposium, St. Paul, MN.
  • Danielle Perry, 1997. "Levels of Rap1A during NIH-3T3 cell proliferation," Minnesota Academy  of Science Annual Meeting, St. Paul, MN.
  • Amanda Plourde, 2008. "Rap's role in the PI3K signaling pathway." Minnesota Academy of Science, Arden Hills, MN.
  • Michael Rafferty and J. Cruise. 1996. "Decrease in rap1A expression precedes and persists during S-phase in regenerating rat liver." Keystone Symposium on Small  G Proteins, Tamarron, CO.
  • Ann Ryan, 1997. "Alpha-adrenergic receptor binding in MRC-5 human fibroblasts," Minnesota  Academy of Science Annual Meeting, St. Paul, MN
  • Brian Smoley. 1993. "Detection of ras p21 proteins in rat liver membranes using an enzyme linked immunosorbant assay." North Central Region District 3 Tri Beta Convention, St. Peter, MN
  • Thomas Trempe, 1994. "Cyclic AMP found to inhibit DNA synthesis in female rat hepatocytes." North Central Region District 3 Tri Beta Convention, St. Paul, MN.
  • Amy VanderBloemen, 1992. "The role of GAP-bound p21 in tumor cell proliferation." Minnesota Academy of Science,  St. Paul, MN.
  • Wendy Wagoner,  1992. "Hepatocyte growth regulation effects of insulin and cAMP. "North Central Region Tri Beta Convention, Chicago, IL.
  • Daniel Wenkel, 1992. "Effect of neomycin on norepinephrine-stimulated DNA synthesis in cultured rat hepatocytes." 6th National Conference on Undergraduate Research, Minneapolis, MN
  • Colleen Wherley. 1991. "Sex differences in the stimulated DNA synthesis of rat  hepatocytes in vitro."  Minnesota Academy of Science, St. Paul, MN.

Student Researchers

Current

 

Previous

  •  Kristen Anderson
  • Meghan Black
  • Stephanie Brockman
  • Jessica Broten
  • William Cross
  • Andrea Daniel
  • Dave Fleischacker
  • Dan Fluegel
  • Amy Gaviglio
  • Michelle Gorman
  • Matt Hallman
  • Steve Harrold
  • Justin Herold
  • Maria Hindt
  • Sara Hoper
  • Julie Horbul
  • Keith Hultman
  • Sara Hyatt
  • Jessica Janisch
  • Andrea Johnson
  • Nikos Katopodis
  • Dan Kolar
  • Jennifer Kuzma
  • Brian Landrigan
  • Tinna Laughlin
  • Shirdi Letran
  • Alyson Lokken
  • Karen Lommel
  • Dan Louvar
  • Maddi Mayry
  • Ryan McAdams
  • Maureen McDonnell
  • Colleen Meyer
  • Katie Meyer
  • Rebecca Mooney
  • Naomi Mraz
  • Yvonne  Mudek
  • James Omlie
  • Tami Pham
  • Danielle Perry
  • Amanda Plourde
  • Michael Rafferty
  • Ann Ryan
  • Laura Smid
  • Evan Smith
  • Brian Smoley
  • Angela Streit
  • Thomas Trempe,
  • Amy VanderBloemen
  • Tyler Varhol
  • Wendy Wagoner
  • Daniel Wenkel
  • Colleen Wherley